Assign background

Menu:   Processing > Background > Assign

Script:   bkAssign

Use this command to assign the intensity of a bright field background for transmission and optical density measurements. Both transmission.and optical density use the value of the average intensity of the background bright field which is usually established during the Background correction. If no background correction has been performed, use this command to assign the average background intensity to the image. By default the bright field intensity is set to the maximum possible intensity value for the given image type except for the floating point type that has a default value of the background set to 1.

If you choose this command, the following options will become available:

Image

Displays the frame number of the image to which the background intensity will be assigned. If you want to assign the background value to another image, type or select the corresponding value.

Intensity

Lets you enter the value of the average bright field intensity that will be used in transmission and optical density measurements.