Track background  
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Menu:   Processing > Background > Track  
Script:   bkTrack  

Use this command to automatically remove or extract background from a grayscale or color image. This is especially useful when applied to an image in which dark or bright objects of similar size are located on an uneven background. The function works by removing pixels corresponding to objects of interest and replacing them with the average background level in the vicinity of the objects.
If you choose this command, the following options will become available:

Displays the input image frame number. If you want to convert another image, type or select the corresponding value.  

Displays the number of the frame in which the output image will be created. Depending on the Preferences. ImageWarp will set it either to the first available value or to the Input frame number. Type or select another value if you want the output image to be created in a different frame.  

Select this check box to watch the Preview of the output image.  
Lets you select the size of the averaging window. This parameter should be in the range of the maximum linear dimension of the largest objects in the image.  

Lets you indicate the minimum intensity difference between the objects and background. Small background variations that fall beneath this level will not be eliminated.  

Lets you choose the intensity of the background relative to the foreground objects.  
Click Dark if the image contains bright objects on a dark field.  
Click Bright if the image contains dark objects on a bright field.  
Allows you to choose the mode for the operation:  
Click Remove to remove the detected background from the image.  
Click Select to extract the detected background .