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Menu:   Processing > Morphology… > Basic->BottomHat  
Script:   bottomHat  
Use this command to detect small dark regions in an image. The bottom-hat transformation also known as "well" or "rolling ball" works by subtracting the original image from the result of the Close
.operator. The shape and size of the neighborhood pattern which could be interpreted as the "brim of the hat" is selected with the Probe option. This function is typically followed by Thresholdng
in order to extract those pits that fall into a specific range of intensity depth.

If you choose this command, the following options will become available:

Displays the input image frame number. If you want to perform the operation on another image, type or select the corresponding value.  

Displays the number of the frame in which the output image will be created. Depending on the Preferences. ImageWarp will set it either to the first available value or to the Input frame number. Type or select another value if you want the output image to be created in a different frame.  
Select this check box to watch the Preview of the output image.  
Lets you chose the size and shape of the kernel used in the operation. Probe elements with check marks are active, empty elements are ignored. Choose among the following most usable kernels:  
1x3 vertical bar
3x1 horizontal bar
3x3 cross
3x3 diagonal cross
3x3 square
5x5 circle