Minimize Error  
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Menu:   Processing > Threshold ->Automatic->Minimize error  
Script   threshDistr  

Use this command to automatically separate objects of interest from the background by using the error minimization technique. This method assumes a histogram in which two bell curves (one of light objects and the other of dark objects) overlap. The threshold is set to that which minimizes the classification error at the overlap.

If you choose this command, the following options will become available:

Displays the input image frame number. If you want to apply thresholding to another image, type or select the corresponding value.  

Displays the number of the frame in which the segmented binary image will be created. Depending on the Preferences. ImageWarp will set it either to the first available value or to the Input frame number. Type or select another value if you want the output image to be created in a different frame.  

Lets you select the color channel that will be used for the histogram analysis.  
Click Luminance to separate objects of interests based on the intensity distribution.  
Click Hue to separate objects of interests based on the color pigment distribution.  
Click Saturation to separate objects of interests based on the color saturation distribution.  
This option is disregarded for grayscale images.  
Select this check box to invert the operation. The pixels whose values are equal or above the calculated threshold level will be treated as background ones, while the pixels with values below the will be treated as foreground ones.  
Ignore B&W
Select this check box to exclude saturated black and white pixels (minimum and maximum pixel values for the given type of image) from the calculation.