Area Percent  
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Menu:   Processing > Threshold ->Automatic->Area Percent  
Script   threshPercent  

Use this command to automatically separate objects of interest from the background by dividing an image histogram into two parts based on a specified percentage of background pixels. For color images thresholding can be performed on the luminance, hue or saturation channel. The function creates a binary output image and returns a calculated threshold.

If you choose this command, the following options will become available:

Displays the input image frame number. If you want to apply thresholding to another image, type or select the corresponding value.  

Displays the number of the frame in which the segmented binary image will be created. Depending on the Preferences. ImageWarp will set it either to the first available value or to the Input frame number. Type or select another value if you want the output image to be created in a different frame.  

Lets you select the color channel that will be used for the histogram analysis.  
Click Luminance to separate objects of interests based on the intensity distribution.  
Click Hue to separate objects of interests based on the color pigment distribution.  
Click Saturation to separate objects of interests based on the color saturation distribution.  

Use this parameter to indicate the percentage of background pixels.  
Select this check box to invert the operation. The pixels whose values are equal or above the calculated threshold level will be treated as background ones, while the pixels with values below the will be treated as foreground ones.