Automatic Measurements

Menu:   Measure > Blobs & Fields > Automatic

Script:   measObjects, measFields

Use this command to perform automatic blob and field measurements and collect information about geometrical, optical and topological properties of objects represented by blobs. A blob is an area of connected pixels in the Input image, which can only be of a multiphase type. Such an image is usually created as a result of the segmentation of the original Photo image. ImageWarp takes intensity measurements from the Photo image, using the Input image as a binary mask. You can save the results into an ImageWarp data file and open it later as a data table, or you can display the results immediately in the Global Grid. You can also export the results to Microsoft Excel for further analysis. Before performing blob measurements, make sure your image is calibrated with the proper spatial and intensity scales, and the background is corrected for transmission and optical density measurements..

When you choose this command, the Automatic Measurements dialog box will appear with the Objects and Fields tabs. In the Objects mode ImageWarp will measure individual blobs, while in the Fields mode it will deliver the information about the phases to which the objects belong . Choose the desired type of measurements by activating the corresponding tab and select the measurement parameters from the Measure/Parameters menu.

For the Objects mode choose among the following parameters:

AngleMajor the angle between the major axis of an object and X-axis AngleMinor the angle between the minor axis of an object and X-axis AngleDMax the angle between the maximum diameter of an object and X-asix AngleDMin the angle between the minimum diameter of an object and X-asis AngleFMax the angle between the longest caliper of an object and X-axis AngleFMin the angle between the shortest caliper of an object and X-axis AngleRMax the angle between the maximum radius of an object and X-axis AngleRMin the angle between the minimum radius of an object and X-axis Area the area of an object in calibrated units AreaBox the area of the box bounding an object in calibrated units AreaConv the area of object's convex hull in calibrated units AreaEll the area of the equivalent ellipse of an object in calibrated units AreaOut the area of an object measured by integrating its outside boundary AreaRatio the ratio of the area of a phase to the reference area AreaRef the reference area of the image or selection in calibrated units Aspect the ratio between the major and minor axes of an object AxisMajor the length of the major axis of object's equivalent ellipse in calibrated units AxisMinor the length of the minor axis of object's equivalent ellipse in calibrated units

BlueAvrg the average value of the scaled blue component of an object BlueSD the standard deviation of the blue component within an object BoxLength the length of object's bounding box along its major axis in calibrated units BoxWidth the width of object's bounding box along its minor axis in calibrated units BoxRatio the ratio between the length and width of obejct's bounding box CenterX the X-coordinate of object's binary weighted centroid in calibrated units CenterY the Y-coordinate of object's binary weighted centroid in calibrated units CenterDist the distance between object's centroid and the origin of the image CenterAngle the polar angle of object's centroid CenterConvX the X-coordinate of the binary weighted centroid of object's convex hull CenterConvY the Y-coordinate of the binary weighted centroid of object's convex hull CenterGrayX the X-coordinate of object's intensity weighted center of gravity CenterGrayY the Y-coordinate of object's intensity weighted center of gravity Circularity the circular form-factor of an object Class the class ID of an object ContourX the vector of X-coordinates of the object's contour ContourY the vector of Y-coordinates of the object's contour Convexity the ratio of the outline area and convex area of an object Count the ordinal number of an object in the current session DCircle the diameter of the circle with the same area as object's area Diameters the vector of the diameters of an object passing through its centroid DAvrg the average diameter of an object passing through its centroid DMax the maximum diameter of an object passing through its centroid DMin the minimum diameter of an object passing through its centroid DRatio the ratio between the maximum and minimum diameters of an object

Ellipticity the elliptical form-factor of an object FeretAvrg the average caliper length of an object in calibrated units FeretMax the maximum caliper length of an object in calibrated units FeretMin the minimum caliper length of an object in calibrated units FeretRatio the ratio between the maximum and minimum calipers of an object FeretX the length of the horizontal projection of an object in calibrated units FeretY the length of the vertical projection of an object in calibrated units GrayAvrg the average scaled intensity of an object GrayI the integral scaled intensity of an object GrayMax the maximum scaled intensity within an object GrayMin the minimum scaled intensity within an object GraySD the standard deviation of scaled intensity within an object GreenAvrg the average value of the scaled green component of an object GreenSD the standard deviation of the green component within an object Histogram the vector of pixel intensity distribution within an object Holes the number of holes within an object HoleArea the total area of holes within an object in calibrated units

MaxX the maximum X coordinate of an object in calibrated units MinX the minimum X coordinate of an object in calibrated units MaxY the maximum Y coordinate of an object in calibrated units MinY the minimum Y coordinate of an object in calibrated units Moments the vector of the object's central binary moments OptdAvrg the average optical density of an object OptdI the integral optical density of an object OptdSD the standard deviation of the optical density within an object Perim the length of the boundary of an object in calibrated units PerimCirc the perimeter of a circle with the area equal to the area of an object PerimConv the perimeter of the convex hull of an object in calibrated units PerimEll the perimeter the object's equivalent ellipse in calibrated units PerimOut the length of the outside boundary of an object not including inside PerimX the horizontal component of the length of an object's boundary PerimXY the diagonal component of the length of an object's boundary PerimY the vertical component of the length of an object's boundary Perforation The ratio of the area of holes within an object to its total area Pixels the number of pixels within an object, not calibrated Profile the vector of intensities along the object's contour

RadiiIn the vector of the inner radii of an object RadiiOut the vector of the outer radii of an object RBest the radius of the circle that most closely fits an object's boundary Rectangularity the rectangular form-factor of an object RedAvrg the average value of the scaled red component of an object RedSD the standard deviation of the red component within an object RAvrg the average radius of an object measured from its centroid RMax the maximum radius of an object measured from its centroid RMin the minimum radius of an object measured from its centroid RRatio the ratio between the maximum and minimum radii of an object Roughness the ratio between the outline perimeter and convex perimeter of an object StartX the X-coordinate of the first found point of an object in pixels StartY the Y-coordinate of the first found point of an object in pixels SkewMajor the binary weighted object's skewness along its major axis SkewMinor the binary weighted object's skewness along its minor axis Skew12 the binary weighted asymmetry factor of an object Skew21 the binary weighted asymmetry factor of an object TransmAvrg the average value of the scaled optical transmission of an object TransmSD the standard deviation of the optical transmission within an object YatMaxX the associated Y-value at the maximum X coordinate of an object YatMinX the associated Y-value at the minimum X coordinate of an object XatMaxY the associated X-value at the maximum Y coordinate of an object XatMinY the associated X-value at the minimum Y coordinate of an object

For the Field mode choose among the following parameters:

Anisotropy the spatial anisotropy of a phase Area the area of a phase in calibrated units AreaRef the reference area of the image or selection in calibrated units BlueAvrg the average value of the scaled blue component of a phase BlueSD the standard deviation of the blue component within a phase ChordSum the total length of horizontal grid lines crossing a phase ChordRef the total length of horizontal grid lines within the image or selection Class the class ID of a field Count the ordinal number of a field in the current session GrayAvrg the average scaled intensity of a phase GrayI the integral scaled intensity of a phase GrayMax the maximum scaled intensity within a phase GrayMin the minimum scaled intensity within a phase GraySD the standard deviation of scaled intensity within a phase GreenAvrg the average value of the scaled green component of a phase GreenSD the standard deviation of the green component within a phase Histogram the vector of pixel intensity distribution within a phase Holes the number of holes within a phase HoleArea the total area of holes within a phase InterceptX the number of intercections of the horizontal grid with phase boundaries InterceptY the number of intercections of the vertical grid with phase boundaries OptdAvrg the average optical density of a phase OptdI the integral optical density of a phase OptdSD the standard deviation of the optical density within a phase Perim the length of the boundary of a phase in calibrated units PerimOut the length of the outside boundary of an object not including inside PerimX the horizontal component of the length of an object or phase boundary PerimXY the diagonal component of the length of a phase boundary PerimY the vertical component of the length of a phase boundary Perforation The ratio of the area of holes within a phase to its total area PhaseRatio the ratio of the area of a phase to the reference area Pixels the number of pixels within a phase, not calibrated RedAvrg the average value of the scaled red component of a phase RedSD the standard deviation of the red component within a phase TransmAvrg the average value of the scaled optical transmission of a phase TransmSD the standard deviation of the optical transmission within a phase

The Data file field should contain the name of the data file that will receive the results of measurements. If you leave this field empty, the results will be sent to the Global Data table and will be displayed immediately after the execution of the function.

The following options are available in the Automatic Measurements dialog box:

Input

Displays the number of the multiphase image frame for which the measurements are taken. If you want to perform measurements of another image, type or select the corresponding value.

Photo

Displays the number of the image frame used for intensity measurements. This is usually an original real-color or grayscale image from which the Input image was derived as a result of image processing and segmentation. When measuring intensity parameters, ImageWarp treats the Input image as a binary mask for the Photo image. If you do not need to perform the intensity measurements, you can set this value to the number of the Input frame and work with the multiphase image only.

Data file

Lets you select or enter the data file to which the results will be written. When you click this button, the Open dialog box will appear allowing you to select the data file from the list of files and folders. If a file of the same name already exists, ImageWarp will append it with the new measurement data. In this case the set of parameters from the data file will take precedence over the set selected from the menu. If you leave this field empty, ImageWarp will collect the results in memory and display them in the Global Grid.

OK

Performs measurements of selected parameters and writes results to the data file or global table.

Cancel

Closes the Automatic Measurements dialog box without performing any measurements.